Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes
Identifieur interne : 000261 ( France/Analysis ); précédent : 000260; suivant : 000262Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes
Auteurs : S. Villechanoux [France] ; Monique Garnier [France] ; Joël Renaudin [France] ; M. Bové [France]Source :
- Current Microbiology [ 0343-8651 ] ; 1992-02-01.
Abstract
Abstract: Greening disease of citrus is caused by a phloem-restricted, bacterium-like organism (BLO). DNA was purified from phloem tissue of periwinkle plants infected with an indian strain of the greening BLO, restricted withHindIII endonuclease, and cloned in the replicative form of bacteriophage M13mp18. By differential hybrizations involving DNA from healthy and infected periwinkle plants, we have selected three recombinant phages containing BLO DNA. The BLO DNA inserts (In-2.6, In-1.9, and In-0.6) have been purified from the viral replicative forms and used as probes. Southern and dot hybridizations have shown that In-2.6 and In-1.9 recognized all asian strains tested (strains from India, Thailand, the Philippines, Indonesia, China, and Taiwan), but not a South African strain. In-0.6 reacted only with the indian BLO strain.
Url:
DOI: 10.1007/BF01570903
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001528
- to stream Istex, to step Curation: 001527
- to stream Istex, to step Checkpoint: 001163
- to stream Main, to step Merge: 003D14
- to stream Main, to step Curation: 003A06
- to stream Main, to step Exploration: 003A06
- to stream France, to step Extraction: 000261
Links to Exploration step
ISTEX:758ED90F1434C52AD2570DB8711E7E19A7E9B49DLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes</title><author><name sortKey="Villechanoux, S" sort="Villechanoux, S" uniqKey="Villechanoux S" first="S." last="Villechanoux">S. Villechanoux</name></author><author><name sortKey="Garnier, Monique" sort="Garnier, Monique" uniqKey="Garnier M" first="Monique" last="Garnier">Monique Garnier</name></author><author><name sortKey="Renaudin, Joel" sort="Renaudin, Joel" uniqKey="Renaudin J" first="Joël" last="Renaudin">Joël Renaudin</name></author><author><name sortKey="Bove, M" sort="Bove, M" uniqKey="Bove M" first="M." last="Bové">M. Bové</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:758ED90F1434C52AD2570DB8711E7E19A7E9B49D</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1007/BF01570903</idno><idno type="url">https://api.istex.fr/document/758ED90F1434C52AD2570DB8711E7E19A7E9B49D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001528</idno><idno type="wicri:Area/Istex/Curation">001527</idno><idno type="wicri:Area/Istex/Checkpoint">001163</idno><idno type="wicri:doubleKey">0343-8651:1992:Villechanoux S:detection:of:several</idno><idno type="wicri:Area/Main/Merge">003D14</idno><idno type="wicri:Area/Main/Curation">003A06</idno><idno type="wicri:Area/Main/Exploration">003A06</idno><idno type="wicri:Area/France/Extraction">000261</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes</title><author><name sortKey="Villechanoux, S" sort="Villechanoux, S" uniqKey="Villechanoux S" first="S." last="Villechanoux">S. Villechanoux</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux, Domaine de la Grande Ferrade, B.P. 81, 33883, Villenave D'Ornon Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave D'Ornon</settlement></placeName><orgName type="university">Université de Bordeaux</orgName></affiliation></author><author><name sortKey="Garnier, Monique" sort="Garnier, Monique" uniqKey="Garnier M" first="Monique" last="Garnier">Monique Garnier</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux, Domaine de la Grande Ferrade, B.P. 81, 33883, Villenave D'Ornon Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave D'Ornon</settlement></placeName><orgName type="university">Université de Bordeaux</orgName></affiliation></author><author><name sortKey="Renaudin, Joel" sort="Renaudin, Joel" uniqKey="Renaudin J" first="Joël" last="Renaudin">Joël Renaudin</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux, Domaine de la Grande Ferrade, B.P. 81, 33883, Villenave D'Ornon Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave D'Ornon</settlement></placeName><orgName type="university">Université de Bordeaux</orgName></affiliation></author><author><name sortKey="Bove, M" sort="Bove, M" uniqKey="Bove M" first="M." last="Bové">M. Bové</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux, Domaine de la Grande Ferrade, B.P. 81, 33883, Villenave D'Ornon Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Nouvelle-Aquitaine</region><region type="old region" nuts="2">Aquitaine</region><settlement type="city">Villenave D'Ornon</settlement></placeName><orgName type="university">Université de Bordeaux</orgName></affiliation></author></analytic><monogr></monogr><series><title level="j">Current Microbiology</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Current Microbiology</title><idno type="ISSN">0343-8651</idno><idno type="eISSN">1432-0991</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>New York</pubPlace><date type="published" when="1992-02-01">1992-02-01</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="89">89</biblScope><biblScope unit="page" to="95">95</biblScope></imprint><idno type="ISSN">0343-8651</idno></series><idno type="istex">758ED90F1434C52AD2570DB8711E7E19A7E9B49D</idno><idno type="DOI">10.1007/BF01570903</idno><idno type="ArticleID">BF01570903</idno><idno type="ArticleID">Art5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0343-8651</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Greening disease of citrus is caused by a phloem-restricted, bacterium-like organism (BLO). DNA was purified from phloem tissue of periwinkle plants infected with an indian strain of the greening BLO, restricted withHindIII endonuclease, and cloned in the replicative form of bacteriophage M13mp18. By differential hybrizations involving DNA from healthy and infected periwinkle plants, we have selected three recombinant phages containing BLO DNA. The BLO DNA inserts (In-2.6, In-1.9, and In-0.6) have been purified from the viral replicative forms and used as probes. Southern and dot hybridizations have shown that In-2.6 and In-1.9 recognized all asian strains tested (strains from India, Thailand, the Philippines, Indonesia, China, and Taiwan), but not a South African strain. In-0.6 reacted only with the indian BLO strain.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Villenave D'Ornon</li></settlement><orgName><li>Université de Bordeaux</li></orgName></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Villechanoux, S" sort="Villechanoux, S" uniqKey="Villechanoux S" first="S." last="Villechanoux">S. Villechanoux</name></region><name sortKey="Bove, M" sort="Bove, M" uniqKey="Bove M" first="M." last="Bové">M. Bové</name><name sortKey="Garnier, Monique" sort="Garnier, Monique" uniqKey="Garnier M" first="Monique" last="Garnier">Monique Garnier</name><name sortKey="Renaudin, Joel" sort="Renaudin, Joel" uniqKey="Renaudin J" first="Joël" last="Renaudin">Joël Renaudin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000261 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000261 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:758ED90F1434C52AD2570DB8711E7E19A7E9B49D
|texte= Detection of several strains of the bacterium-like organism of citrus greening disease by DNA probes
}}
| This area was generated with Dilib version V0.6.25. |  |